Dr. Attila Ambrus

Assistant Professor and Senior Research Fellow of the Hungarian Academy of Sciences

Semmelweis University, Department of Medical Biochemistry

Publications

  1. Ambrus A, Fésüs L, Polyethylene glycol enhanced refolding of the recombinant human tissue transglutaminase. Prep. Biochem. Biotechnol. 31(1): 59-70 (2001)
  2. Ambrus A, Banyai I, Weiss M, Hilgenfeld R, Keresztessy Z, Muszbek L, Fesus L, Calcium binding of transglutaminases: a 43Ca NMR study combined with surface polarity analysis. J. Biomol. Struct. Dyn. 19(1): 59-74 (2001)
  3. Ambrus A, Batta G, Kövér K, Groves P, Palczewska M, Kuznicki J, An NMR study of calretinin, a calcium binding protein. The first two domains. Biokémia (Hungarian) 25(2): 37-39 (2001)
  4. Palczewska M, Groves P, Ambrus A, Kaleta A, Kover K, Batta G, Kuznicki J, Structural and biochemical characterization of neuronal calretinin domain I-II (residues 1-100). Comparison to homologous calbindin D28k domain I-II (residues 1-93). Eur. J. Biochem. 268(23): 6229-37 (2001)
  5. Roberts S, Wildner G, Grass G, Weichsel A, Ambrus A, Rensing C, Montfort W, A labile regulatory copper ion lies near the T1 copper site in the multicopper oxidase CueO. J. Biol. Chem. 278 (34): 31958-63 (2003)
  6. Ambrus A, Chen D, Dai J, Jones R, Yang D, Solution structure of the biologically relevant G-quadruplex element in the human c-MYC promoter. Implications for G-quadruplex stabilization. Biochemistry-US 44 (6): 2048-2058 (2005) [featured on the cover of Biokémia 31(1), 2007]
  7. Ambrus A, Chen D, Whatcott C, Somogyi A, Yang D, Matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry protocol for monitoring the progress of enzymatic C-13/N-15-labeled DNA syntheses. Anal.  Biochem. 342 (2): 246-53 (2005)
  8. Tangirala R, Dixon R, Yang D, Ambrus A, Antony S, Agama K, Pommier Y, Curran D, Total and semisynthesis and in vitro studies of both enantiomers of 20-fluorocamptothecin. Bioorg. Med. Chem. Lett. 15(21): 4736-40 (2005)
  9. Dai J, Dexheimer T, Chen D, Carver M, Ambrus A, Jones R, Yang D, An intramolecular G-quadruplex structure with mixed parallel/antiparallel G-strands formed in the human Bcl-2 promoter region in solution. J. Am. Chem. Soc. 128 (4): 1096-98 (2006) [ISI Web of Knowledge Essential Science Indicators Highly Cited Paper]
  10. Ambrus A, Friedrich K, Somogyi A, Oligomerization of nitrophorins. Anal. Biochem. 352 (2): 286-95 (2006)
  11. Ambrus A, Chen D, Dai J, Bialis T, Jones R, Yang D, Human telomeric sequence forms a hybrid-type intramolecular G-quadruplex structure with mixed parallel/antiparallel strands in potassium solution. Nucleic Acids Res. 34 (9): 2723-35 (2006) [ISI Web of Knowledge Essential Science Indicators Highly Cited Paper; featured in Chemical & Engineering News (C&EN, ACS), July 31, 2006]
  12. Dai J, Punchihewa C, Ambrus A, Chen D, Jones R, Yang D, Structure of the intramolecular human telomeric G-quadruplex in potassium solution: A novel adenine triple formation. Nucleic Acids Res. 35(7): 2440-50 (2007) [featured on the cover of Chemical & Engineering News (C&EN, ACS), May 28, 2007]
  13. Ambrus A, Yang D, Diffusion ordered NMR spectroscopy for analysis of DNA secondary structural elements. Anal. Biochem. 367(1):56-67 (2007)
  14. Ambrus A, G-quadruplexes: structure and biological significance. Biokémia (Hungarian) 31(1): 2-8 (2007)
  15. Ambrus A, Torocsik B, Adam-Vizi V, Periplasmic cold expression and one-step purification of human dihydrolipoamide dehydrogenase. Protein Expr. Purif., 63(1): 50-57 (2009)
  16. Vajda S, Mandi M, Konrad C, Kiss G, Ambrus A, Adam-Vizi V, Chinopoulos C, A re-evaluation of the role of matrix acidification in uncoupler-induced Ca2+-release from mitochondria. FEBS J. 276(10): 2713-2724 (2009)
  17. Ambrus A, Torocsik B, Adam-Vizi V, Refolding of the human dihydrolipoamide dehydrogenase. Biochem. Eng. J. 45(2): 120-125 (2009)
  18. Ambrus A#, Tretter L#, Adam-Vizi V, Inhibition of the alpha-ketoglutarate dehydrogenase-mediated reactive oxygen species generation by lipoic acid. J. Neurochem. 109(S1): 222-229 (2009) (#=contributed equally)
  19. Dai J, Ambrus A, Hurley L, Yang D, A direct and nondestructive approach to determine the folding structure of the i-motif DNA secondary structure by NMR. J. Am. Chem. Soc. 131(17): 6102-6104 (2009)
  20. Ambrus A, Torocsik B, Tretter L, Ozohanics O, Adam-Vizi V, Stimulation of reactive oxygen species generation by disease-causing mutations of lipoamide dehydrogenase, Hum. Mol. Genet. 20(15): 2984-2995 (2011)
  21. Ambrus A, Adam-Vizi V, Molecular dynamics study of the structural basis of dysfunction and the modulation of reactive oxygen species generation by pathogenic mutants of human dihydrolipoamide dehydrogenase, Arch. Biochem. Biophys. 538(2):145-155 (2013)
  22. Tretter L, Ambrus A, Measurement of ROS homeostasis in isolated mitochondria, Method. Enzymol. 547: 199–223 (2014)
  23. Nemeria NS#, Ambrus A#, Patel H#, Gerfen G, Adam-Vizi V, Tretter L, Zhou J, Wang J, Jordan F, Human 2-oxoglutarate dehydrogenase complex E1 component forms a thiamin-derived radical by aerobic oxidation of the enamine intermediate, J. Biol. Chem. 289: 29859-29873 (2014) (#=contributed equally)
  24. Ambrus A, Mizsei R, Adam-Vizi V, Structural alterations by five disease-causing mutations in the low-pH conformation of human dihydrolipoamide dehydrogenase (hLADH) analyzed by molecular dynamics – implications in functional loss and modulation of reactive oxygen species generation by pathogenic hLADH forms, Biochem. Biophys. Reports, 2:50-56 (2015)
  25. Ambrus A, Nemeria NS, Torocsik B, Tretter L, Nilsson M, Jordan F, Adam-Vizi V, Formation of reactive oxygen species by human and bacterial pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes reconstituted from recombinant components, Free Radic. Biol. Med. 89: 642-650 (2015)
  26. Harami-Papp H, Pongor SL, Munkácsy G, Horvath G, Nagy MA, Ambrus A, Hauser P, Szabo A, Tretter L, Gyorffy B, TP53 mutation hits energy metabolism and increases glycolysis in breast cancer, Oncotarget, 7:67183-67195 (2016)
  27. Ambrus A#, Wang J#, Mizsei R#, Zambo Z, Torocsik B, Jordan F, Adam-Vizi V, Structural alterations induced by ten disease-causing mutations of human dihydrolipoamide dehydrogenase analyzed by hydrogen/deuterium-exchange mass spectrometry: Implications for the structural basis of E3 deficiency, Biochim. Biophys. Acta (Molecular Basis of Disease) 1862: 2098-2109 (2016) (#=contributed equally) (invited paper to Ambrus A)

  28. Szabo E, Ambrus A, Molecular pathomechanism of the human dihydrolipoamide dehydrogenase deficiency, Biokémia (Hungarian), 41(2): 44-63 (2017) 
  29. Ambrus A, Adam-Vizi V, Human dihydrolipoamide dehydrogenase (E3) deficiency:novel insights into the structural basis and molecular pathomechanism, Neurochem. Int., accepted, in press, https://doi.org/10.1016/j.neuint.2017.05.018 (2018)
  30. Kiss E, Mirzahosseini A, Hubert A, Ambrus A, Orfi L, Horvath P, DNA binding of Sunitinib: spectroscopic evidence via circular dichroism and nuclear magnetic resonance, J. Pharm. Biomed. Anal. 150: 355-361 (2018)

  31. Mikulas K, Hermann P, Gera I, Komlódi T, Horváth G, Ambrus A, Tretter L, Triethylene glycol dimethacrylate impairs bioenergetic functions and induces oxidative stress in mitochondria via inhibiting respiratory Complex I, Dent. Mater., accepted, in press (2018)

     

Google Scholar: http://scholar.google.com/citations?user=hKVjrQcAAAAJ&hl=en

MTMT: https://vm.mtmt.hu//search/slist.php?lang=0&AuthorID=10000177

ORCID: http://orcid.org/0000-0001-6014-3175